Figure 3.

Effects of quaking homologue (QKI)‐6 overexpression and knockdown on regulating bladder cancer cell growth, clone formation and invasion. (A‐C), Western blot and qRT‐PCR. QKI mRNA and protein expression were assessed using Western blot and qRT‐PCR in bladder cancer cell lines 5637 and T24 after infection with lentivirus carrying QKI shRNA or cDNA, respectively. (D‐E), Cell viability CCK‐8 assay. Bladder cancer cell lines 5637 and T24 were infected with lentivirus carrying QKI shRNA, QKI cDNA or their negative controls, respectively, and then subjected to CCK‐8 assay. Cell growth curves are based on average absorbance values (n = 6). (F), Immunofluorescence. Bladder cancer cell lines 5637 and T24 were infected with lentivirus carrying QKI shRNA, QKI cDNA or their negative controls, respectively, and then subjected to immunofluorescence analysis of Ki‐67 expression (400×). (G), Colony formation assay. Duplicated cells were subjected to the tumour cell colony formation assay. (H), Quantified data of G. (I), Transwell invasion assay. Bladder cancer cell lines 5637 and T24 were infected with lentivirus carrying QKI shRNA, QKI cDNA or their negative controls, respectively, and then subjected to Transwell invasion assay (400×). (J), Quantified data of I. All assays were performed at least three times independently and error bars represent the mean ± SD and one‐way ANOVA analysis of three independent experiments. *P < 0.05