Figure 4.

The effects of MEG3 on the growth of laryngeal cancer cells in vivo. Hep‐2 cells transfected with empty vector or MEG3 plasmid were injected into nude mice (n = 4/group). A, Tumour volumes were measured every 3 d after injection. At 15 d after injection, xenograft tumours were harvested and weighed. B, Immunohistochemistry assay was performed to assess the protein levels of Ki67 and cleaved caspase‐3. Magnification: ×400, scale bars: 50 μm. C, Western blotting was employed to measure protein level of cleaved caspase‐3 in xenograft tumour tissues. The data were obtained from three independent experiments and presented as mean ± SEM, ** P < .01