Figure 3.

miR‐127‐5p could bind to MMP‐13 and suppress its expression. A, The targeted relationship between miR‐127‐5p and MMP‐13 using Circular RNA Interactome (https://circinteractome.nia.nih.gov/ ) and it was verified by dual‐luciferase reporter gene assay. B, The expression of miR‐127‐5p could be up‐regulated with miR‐127‐5p mimics yet down‐regulated with miR‐127‐5p inhibitor. C, Overexpression of miR‐127‐5p greatly down‐regulated the protein expression of MMP‐13, which could be instead up‐regulated with miR‐127‐5p inhibitor. D, E, Relative expression of miR‐127‐5p and MMP‐13 was tested in the normal cartilage tissues and OA cartilage tissues. F, RNA in situ hybridization reveals the immune‐localization of Circ_0136474 and MMP‐13 in OA tissues. Nuclei were stained with DAPI. Scale bar, 10 μm. G, The expression of MMP‐13 was tested by qRT‐PCR and Western blot. **P < 0.01 meant that there stood a significant difference compared with NC group