Figure 3.

Construction of P53 re‐expression KP cells and identification of lncRNAs regulated by P53. A, P53 was efficiently re‐expressed in the primary KP cells. The P53‐re‐expressed KP cells (KP‐P53) were developed by PCDH‐Flag‐P53 lentiviral infection and puromycin selection （4 μg/mL）, using PCDH‐Flag empty vector lentivirus as negative control. Western blot analysis was used to analyse the expression of P53 in lysates from KP cells. B, Comparison between microarray data and qPCR results of lncRNAs regulated by P53. Among the selected 210 lncRNAs, qPCR results determined 11 lncRNAs, whose expression was altered by the re‐expression of P53 in KP cells and exhibited opposite expression alteration direction to that in the microarray of the mouse lung adenocarcinoma tissues. The heights of the columns in the chart represent the log‐transformed median fold changes (tumour sample/normal tissues in microarray data or KP‐p53 cells/KP‐NC cells in qPCR results). Mean ± SD are shown, n = 3 or 2