Table 1.
Hit identification for sublibrary A.
| Compound | First experiment | Second experiment | Third experiment | |||
|---|---|---|---|---|---|---|
| Total binding (normalized area) | Non-specific binding (normalized area) | Total binding (normalized area) | Non-specific binding (normalized area) | Total binding (normalized area) | Non-specific binding (normalized area) | |
| 4-(4-chlorophenyl)-piperidin-4-ol | n.d | n.d | n.d | n.d | n.d | n.d |
| 8-OH DAPT | 0.177 ± 0.006 | 0.193 ± 0.004 | 0.303 ± 0.012 | 0.283 ± 0.042 | 0.176 ± 0.012 | 0.162 ± 0.027 |
| Chlorpromazine | 6.590 ± 0.156 | 6.370 ± 0.198 | 6.173 ± 0.693 | 5.663 ± 0.597 | 6.147 ± 0.314 | 6.280 ± 0.928 |
| DDPM2330 | 0.771 ± 0.003 | 0.738 ± 0.002 | 0.876 ± 0.048 | 0.813 ± 0.031 | 0.490 ± 0.038 | 0.368 ± 0.021 |
| DDPM2565 | 0.571 ± 0.040 | 0.119 ± 0.006 | 0.799 ± 0.063 | 0.335 ± 0.011 | 0.454 ± 0.012 | 0.069 ± 0.017 |
| Doxepin | 0.705 ± 0.025 | 0.666 ± 0.008 | 0.932 ± 0.064 | 0.827 ± 0.096 | 0.433 ± 0.056 | 0.404 ± 0.084 |
| Fenoterol | 0.041 ± 0.008 | 0.041 ± 0.002 | 0.034 ± 0.002 | 0.030 ± 0.004 | 0.021 ± 0.003 | 0.021 ± 0.002 |
| Ketoprofen | 0.004 ± 0.000 | 0.005 ± 0.000 | 0.003 ± 0.000 | 0.003 ± 0.000 | 0.004 ± 0.000 | 0.004 ± 0.001 |
| Meclozine | 4.937 ± 0.861 | 6.095 ± 0.205 | 5.307 ± 0.931 | 5.253 ± 1.007 | 5.188 ± 0.271 | 5.523 ± 0.934 |
| Metoclopramide | 0.370 ± 0.013 | 0.390 ± 0.028 | 0.503 ± 0.027 | 0.457 ± 0.054 | 0.317 ± 0.017 | 0.281 ± 0.042 |
| Oxazepam | 0.132 ± 0.001 | 0.130 ± 0.026 | 0.120 ± 0.020 | 0.111 ± 0.007 | 0.097 ± 0.014 | 0.106 ± 0.024 |
| Piroxicam | 0.054 ± 0.000 | 0.060 ± 0.005 | 0.034 ± 0.001 | 0.034 ± 0.003 | 0.040 ± 0.001 | 0.037 ± 0.004 |
| Procaine | 0.073 ± 0.003 | 0.074 ± 0.006 | 0.092 ± 0.003 | 0.082 ± 0.010 | 0.073 ± 0.005 | 0.063 ± 0.008 |
| Roxithromycin | 0.020 ± 0.003 | 0.021 ± 0.001 | 0.003 ± 0.001 | 0.003 ± 0.001 | 0.012 ± 0.001 | 0.010 ± 0.001 |
| Sulpiride | 0.054 ± 0.002 | 0.056 ± 0.004 | 0.068 ± 0.002 | 0.061 ± 0.009 | 0.055 ± 0.003 | 0.047 ± 0.003 |
| Telmisartan | 3.853 ± 0.130 | 3.405 ± 0.106 | 2.577 ± 0.326 | 2.290 ± 0.340 | 2.257 ± 0.122 | 2.183 ± 0.455 |
n.d., not determined.
Total and nonspecific binding of each sublibrary component at a concentration of 1 μM (three experiments, each performed in triplicates, nonspecific binding determined in presence of 100 mM GABA). Reported values represent means ± SD. Values marked gray represent identified hits based on significantly higher total than nonspecific binding (one-tailed t-test, CL = 97.5%).