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. 2019 Oct 4;13:227. doi: 10.3389/fnbeh.2019.00227

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Copyright © 2019 Moore, Oelberg, Glass, Johnson, Been and Meisel.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Double immunolabeling revealed a significant increase in activated inputs to the NAc core from the prelimbic region of the mPFC. (A) Illustration of the unilateral infusion of cholera toxin B (CTB) targeted to the core of the NAc. (B) Immunohistochemical labeling of the retrograde tracer CTB confirmed injection sites were biased to the core of the NAc. (C) There was no significant change in c-Fos labeling in CTB cells within the total mPFC, though (D) there was a significant increase in c-Fos labeling within CTB cells in the prelimbic (PrL) subregion following sex behavior. (E) The infralimbic (IL) region of the mPFC did not exhibit a change in c-Fos within CTB labeled cells following sex (*p < 0.05).