Figure 1.

Mild hypothermia protects against ROT‐induced apoptosis in SH‐SY5Y neuroblastoma cells. Cells were pre‐cultured under normothermic (37°C) or mild hypothermic (32°C) conditions for 1 d and treated with 0.5 μmol/L ROT. A, An MTT assay was performed to examine the effects of mild hypothermia on cytotoxicity after ROT treatment for a time course of 0, 24, 36 and 48 h. B, Cells were treated with ROT for 24 h, after which Western blotting of cleaved (cl.) PARP, Bcl‐2, Bax, RBM3 and β‐actin was performed. C, D, The levels of cl. PARP and Bcl‐2 were quantified by densitometry and normalized to β‐actin and Bax, respectively. E, TUNEL staining after 24 h of ROT treatment evaluated the protective effects of mild hypothermia on ROT‐induced apoptosis. All data are representative of three independent experiments, and values are mean ± SD.; **P < 0.01 and ***P < 0.001 vs normothermia pre‐cultured cells