Figure 2.

RBM3 protects against ROT‐induced apoptosis. SH‐SY5Y cells were transfected with the empty vector (Veh) or the RBM3‐expressing vector (RBM3) and treated with 0.5 μmol/L ROT. A, An MTT assay was performed to examine the effects of RBM3 overexpression ROT cytotoxicity after ROT treatment for a time course of 0, 24, 36 and 48 h. B, Cells were treated with ROT for 24 h before Western blotting for the indicated proteins. The black and red arrows indicate endogenous RBM3 and myc‐tagged RBM3, respectively. C, D, The levels of cl. PARP and Bcl‐2 were quantified by densitometry and normalized to β‐actin and Bax, respectively. E, TUNEL staining was performed after 24 h ROT exposure to evaluate the protective effects of RBM3 overexpression on ROT‐induced neurotoxicity. ***P < 0.001 vs vehicle‐transfected cells