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TFDG inhibits the hydrolytic activity of MBLs. Nitrocefin was incubated with four β‐lactamase variants pretreated with various concentrations of 32 μg/mL TFDG, after which the samples were measured at OD492 nm. Bars represent the standard deviation (** indicates P < .01 compared with the control group; two‐tailed Student's test)
