Figure 4.

Genetic disruption of sEH promotes the degradation of ABCA1 protein in oxLDL‐treated macrophages. A, Total RNA was isolated at 6 h after oxLDL incubation and ABCA1 and GAPDH mRNA was determined by semi‐quantitative RT‐PCR analysis. B, Macrophages were incubated in the presence of 2 µg/mL cycloheximide (CHX) to inhibit protein translation with oxLDL (50 µg/mL) for the time periods indicated. Data shown are mean ± SD from 5 independent experiments. *P < 0.05 vs oxLDL‐treated WT macrophages