Figure 5.

Role of sEH phosphatase activity in ABCA1 expression and phosphorylation in oxLDL‐treated macrophages. A and B, Macrophages were pre‐treated with 10 µM AUDA (an inhibitor for hydrolase activity of sEH) or ebselen (an inhibitor for phosphatase activity of sEH) for 2 h and then oxLDL (50 µg/mL) for additional 24 h. A, Western blot analysis of ABCA1 and α‐tubulin. B, Cholesterol efflux was measured in the presence of apoAI. C and D, Intracellular cholesterol and lipid accumulation were evaluated by the colorimetric assay kit and oil red O staining. Data are mean ± SD from 5 independent experiments. *P < 0.05 vs vehicle group, # P < 0.05 vs oxLDL‐only group