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Experimental design to determine the effect of various process parameters on the performance of bovine serum albumin (BSA) loaded alginate nanospheres.
| Factors Tested | Alginate Concentration (%) | Cross-Linking Time (min) | Drying Time (h) |
|---|---|---|---|
| 1st Step: Concentration Effect | 3 | 10 | 0 |
| 5 | 10 | 0 | |
| 2nd Step: Cross-linking Time Effect | 5 | 1 | 0 |
| 5 | 10 | 0 | |
| 5 | 30 | 0 | |
| 3rd Step: Drying Time Effect | 5 | 10 | 0 |
| 5 | 10 | 0.5 | |
| 5 | 10 | 1.5 | |
| 5 | 10 | 4.5 | |
| 5 | 10 | 24 |
