Table 2.
Results of the analysis performed by Ion Reporter for the expected variants in cell MIX C at the lowest VAF tested.
| Returned Variant Frequency (%) | p Value | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Gene | Locus | Change | Exp VAF (%) | C1 | C1b | C2 | C2b | C 1 | C 1b | C2 | C 2b |
| BRAF | chr7:140453136 | p.Val600Glu | 1.8 | 2.25 | 1.76 | 1.77 | 2.16 | 0.01338 | 0.1804 | 0.22338 | 0.01236 |
| CDKN2A | chr9:21971177 | p.Glu61Ter | 1.8 | 1.31 nocall | 1.76 | REF | REF | 0.33961 | 0.14022 | ||
| NRAS | chr1:115256529 | p.Gln61Arg | 1.8 | 2.24 | 1.35 nocall | 1.55 nocall | 2.05 | 0.00596 | 0.28338 | 0.42203 | 0.02571 |
| PIK3CA | chr3:178936091 | p.Glu545Lys | 1.8 | 2.06 | 2.92 * | REF | 1.81 | 0.04845 | 0.00001 * | 0.11086 | |
| SMAD4 | chr18:48586291 | c.955 + 5G > C (intronic) | 1.8 | 1.74 | 1.70 | 1.82 | REF | 0.17259 | 0.1884 | 0.12453 | |
| APC | chr5:112175363 | p.Ala1358Thr | 1.8 | REF | REF | REF | REF | ||||
| EGFR | chr7:55242464 | p.Glu746_Ala750del | 1.8 | 1.53 nocall | 1.68 | 2.20 | 2.15 | 0.4187 | 0.22954 | 0.00573 | 0.01061 |
| KRAS | chr12:25398284 | p.Gly12Val | 3.6 | 4.37 * | 4.05 * | 5.42 * | 4.86 * | 0.00001 * | 0.00001 * | 0.00001 * | 0.00001 * |
* Low-allele frequency variants called by Ion Reporter with high significant statistical confidence.