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. 2019 Jul 17;5(3):63. doi: 10.3390/jof5030063

Figure 1.

Figure 1

Gating strategy: Flow cytometry analysis of cryptococcal-specific CD4+ and CD8+ T cell memory and phenotypes evaluated in a 6 h assay: (A) We first used a singlet gate to exclude doublets by gating on forward scatter-area (FSC-A) against forward scatter-height (FSC-H). (B) Lymphocytes were then selected using a forward scatter-area (FSC-A) against side scatter-area (SSC-A) gate. (C) Conventional T cells were selected by gating on CD3+ cells from the total lymphocyte population, which were further divided to (D) CD4+ and CD8+ T cells. Memory phenotypes were assessed by variable expression of CD45R0 and CD27 on the CD4+ (E) and CD8+ (I) T cell populations; while T cell activation was assessed by expression of CD38 and HLA-DR on the CD4+ (G) and CD8+ (H) T cell subsets. T cell exhaustion was assessed by expression of PD-1 (F) on both populations. CM represents cryptococcal meningitis. QuaradntDEM.