Table 1.
DNA sequences of probes and primers used in this study.
Oligonucleotide | Sequence |
---|---|
DENV-1 probe | 5′-GGGAAGCTGTATCCTGGTGGTAAGG(A)14-3′-biotin |
Reporter probe | Thiol-5′-GATGCAAGGTCGCATATGAG-3′ |
Control probe | Biotin-5′-CTCATATGCGACCTTGCATC-3′ |
Primer 1 [a] | 5′-aattctaatacgactcactatagggGAGACAGCAGGATCTCTGGTCT-3′ |
Primer 2 [b] | 5′-gatgcaaggtcgcatatgagGGTTAGAGGAGACCCCTCCC-3′ |
[a] Lowercase bases represent T7 promoter sequence. [b] Lowercase bases are homologous to reporter probe.