Figure 5.

Number of contigs (nonchimeric, chimeric or unidentified) and genes along filtering of the Drosophila melanogaster de novo transcriptome. (a) Flowchart of the number of chimera, correct, and unidentified sequences filtering by Bellerophon. Numbers in black boxes are the number of sequences present in the assembly at the different stages. Numbers for nonchimeric sequences are displayed in violet, in orange for chimeric (orange) or in black for unidentified sequences (sequences which could not be attributed to a gene through a blast).The numbers in black arrows refer to the number of nonchimeric, chimeric, or unidentified sequences removed by each filtering step. The bar charts display the percentage of nonchimeric (violet), chimeric (orange), or unidentified sequences (violet) removed by each filtering step. (b) Number nonchimeric, chimeric, and unidentified contigs along the differents step of the filtering of the de novo D. melanogaster transcriptome with the Bellerophon pipeline. (c) Number of genes represented by at least a contig in the de novo D. melanogaster transcriptome with the Bellerophon pipeline. Number of contigs per gene are display in violet (1 contig only), orange (between 2 and 9 contigs), and in black (10 contigs or more)