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Sequence coverage and entropy for cox2 primers designed by DegePrime based on a trimmed alignment of 3,503 oomycete cox2 sequences. Shown is the sequence coverage of primers along the alignment length with varying degeneracy (1–20) and length (18, 20, 22). Each degeneracy and length combination is displayed in a different color (a). The corresponding entropy for each window position (b). Arrows indicate the position of the chosen reverse primer cox2_233D8r
