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. 2019 Oct 4;10:2319. doi: 10.3389/fmicb.2019.02319

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Copyright © 2019 Whittle, Legood, Alav, Dulyayangkul, Overton and Blair.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Gating strategy for the measurement of ethidium bromide RFU. Panel (A) shows how cells (red) were separated from background debris (black) by gating SYTO^TM 84⁺ cells (red) using a plot of the orange fluorescence emission channel YL1-H (SYTO^TM 84 fluorescence) against forward scatter (FSC-H). (B) Using the red gate of SYTO^TM 84⁺ population, a histogram of ethidium bromide fluorescence per cell was plotted using the emission channel BL3-H. The ethidium bromide gate (blue) gives the x-median of ethidium bromide fluorescence to measure relative accumulation.