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. 2019 Oct 4;10:2319. doi: 10.3389/fmicb.2019.02319

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Copyright © 2019 Whittle, Legood, Alav, Dulyayangkul, Overton and Blair.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Gating strategy when measuring GFP alongside ethidium bromide. As above, SYTO^TM 84⁺ cells were gated above in red (A,D). Panels (A–C) represents strain SL1344 expressing no GFP. (B,E) Plots YL1-H for SYTO^TM 84 against BL1-H for GFP. Only SYTO^TM 84⁺ cells are analyzed in these plots and the GFP⁺ gate (Blue, right hand side) represents cells that are expressing GFP only and excluding cells fluorescing in BL1-H channel by spillover (C,F).