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. 2019 Oct 4;10:2319. doi: 10.3389/fmicb.2019.02319

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Copyright © 2019 Whittle, Legood, Alav, Dulyayangkul, Overton and Blair.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Concentrations of SYTO^TM 84 and ethidium bromide used in this assay. (A) 10 μM SYTO^TM 84 was used and a population gated (R1) on a orange fluorescence (YL1-H) versus forward scatter (FSC-H) dot plot to identify SYTO^TM 84 fluorescence. The concentration was used as it gave the best separation of cells (red) from background cell debris and other particulate noise (black). (B) 100 μM was the optimum concentration of ethidium bromide used as shown via a dot plot of YL1-H (SYTO^TM 84) vs BL3-H (ethidium bromide). Both plots show all events.