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. 2019 Oct 4;10:2319. doi: 10.3389/fmicb.2019.02319

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Copyright © 2019 Whittle, Legood, Alav, Dulyayangkul, Overton and Blair.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Flow cytometry plots of SL1344 and SL1344 ΔacrB. (1) shows plots of SL1344 and (2) shows plots of efflux mutant SL1344 ΔacrB. (A) Separation of cells from background cell debris using channels YL1-H vs. FSC-H. SYTO 84⁺ cells are gated (red gate) with the X-median value given. 10,000 events were collected inside the SYTO 84⁺ gate. From this, the red population is analyzed in (B) plots where the BL3-H channel was used to measure the X-median of ethidium bromide fluorescence in a new gate (EtBr in Blue). Fluorescence of ethidium bromide was only measured from the SYTO 84⁺ population.