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. Author manuscript; available in PMC: 2020 Aug 9.
Published in final edited form as: J Mol Biol. 2019 Jun 4;431(17):3339–3352. doi: 10.1016/j.jmb.2019.05.043

Figure 5. Electrophysiological characterization of mutants at highly conserved positions in TM3b.

Figure 5.

(a) Representative traces of single-substituted mutants of V122 and L118 in E. coli MscS. A scale bar is presented at top left of the traces. Note that absolute pressure values at which channels open differ between patches due to variation in patch geometry. Therefore, an internal reference, MscL channel opening, is used as a reference and the ratio expressed as PL:PS, which is ~1.5 for wild type MscS channels. (b) summary of PL:PS values for the TM3b mutants.