Table 2.
Functional grouping of up- and down-regulated genes in the osteogenic pathway of human MSCs grown on collagen coatings (coating by atmospheric plasma vs. coating by adsorption).
| Function group | Subdivision of function | Gene |
|---|---|---|
| Skeletal development | Cartilage condensation | col2a1, sox9 |
| Ossification | alpl, bglap, col2a1, csf1, fgf2, sox9, tnfsf11 | |
| Osteoclast differentiation | bglap, csf1, tnfsf11 | |
| Osteoblast differentiation | bglap, fgf2 | |
| Other | alpl | |
| Bone mineral metabolism | Bone mineralization | ahsg, bglap, sox9 |
| Calcium ion binding & homeostasis | bglap, comp, fgf2 | |
| Extracellular matrix (ECM) molecules | Collagens | col2a1, col3a1 |
| ECM protease inhibitors | ahsg | |
| ECM Proteases | ctsk, mmp10, phex | |
| Other | alpl, bgn | |
| Cell adhesion molecules | Cell-cell adhesion | col2a1, icam1, sox9, tnfsf11 |
| Cell-ECM adhesion | csf1, itga1 | |
| Other | bglap, | |
| Growth factors | fgf2, gdf10, igf1, vegfa | |
| Transcription factors | sox9 | |
Full list of genes in the human osteogenesis RT2 profiler PCR array can be found on https://www.qiagen.com/gb/shop/pcr/primer-sets/rt2-profiler-pcr-arrays/?catno=PAHS-026Z#geneglobe.