Table 3.
Functional grouping of up- and down-regulated genes in the neurogenic pathway of human NSCs grown on laminin coatings (coating by atmospheric plasma vs. coating by adsorption).
| Function group | Subdivision of function | Gene |
|---|---|---|
| Neuronal migration | ascl1, dcx, cdk5r1, neurog2, nrcam | |
| Cell differentiation | Neuronal differentiation | ascl1, bmp2, cdk5r1, heyl, nrcam, sox2, neurog2 |
| Neuronal cell fate determination | ascl1, sox2 | |
| Other | mdk | |
| Synaptic functions | Regulation of synaptic plasticity | grin1 |
| Synaptic transmission | grin1 | |
| Synaptogenesis | nrcam | |
| Axonogenesis | dcx, map2, nrcam | |
| Growth factors & cytokines | Growth factors | gdnf, mdk, ndp |
| Cytokines | bmp2, mdk | |
| Apoptosis | ep300, gdnf | |
| Cell adhesion molecules | dll1, nrcam | |
| Cell cycle | ep300, mdk | |
| Signal transduction | Notch signaling | ascl1, dll1, heyl |
| WNT signaling | ndp | |
| TGFβ signaling | bmp2 | |
| G-protein coupled receptor signaling | adora2a | |
| Transcription factors & cofactors | ascl1, ep300, heyl, neurog2, sox2, stat3 | |
Full list of genes in the human neurogenesis RT2 profiler PCR array can be found on https://www.qiagen.com/gb/shop/pcr/primer-sets/rt2-profiler-pcr-arrays/?catno=PAHS-404Z#geneglobe.