Figure 1. Strength, fatigue, and signaling pathways associated with protein synthesis in the soleus muscle. A, Parameters of strength and fatigue in the soleus muscle. The results were compared using two-way ANOVA and Bonferroni post hoc test. Aa is P<0.05 for C and Cr groups vs C-HS and Cr-HS groups (main effect of HS), using two-way ANOVA only (no statistical differences using the Bonferroni post-hoc test). B, Markers of signaling pathways associated to protein synthesis in the soleus muscle. Data are reported as means±SE on the basis of total protein loading as indicated by the Ponceau S measurements, n=6–8 animals. The results were compared using two-way ANOVA and Bonferroni post hoc test. Bj is P<0.05 for C and Cr groups vs C-HS and Cr-HS groups (main effect of HS), using two-way ANOVA only (no statistical differences using the Bonferroni post hoc test). C: control group; HS: hindlimb suspension group; Cr: creatine supplemented group; Cr-HS: creatine supplemented and hindlimb suspension group.