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. 2019 Sep 16;42(5):1805–1814. doi: 10.3892/or.2019.7315

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Copyright: © Hah et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — Migration and invasion assay of human skin squamous carcinoma cell lines SCC12 and SCC13. (A) Comparison of the migrational ability of SCC12 and SCC13 cells. A wound was created in a confluent SCC cell monolayer using a sterile micropipette tip, and the cells were incubated for 8 h. Migratory cells in the denuded zone were photographed. Magnification, ×100. (B) Invasion through Matrigel-coated Transwell inserts after 16 h. Nuclei were stained using DAPI, and images of the invaded cells were captured. Compared with SCC13 cells, SCC12 cells exhibited increased migration and invasion capacities, with metastatic potential. Magnification, ×200. SCC, squamous cell carcinoma.