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. 2019 Sep 17;42(5):1689–1698. doi: 10.3892/or.2019.7320

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Copyright: © Yang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — PTEN is downregulated in VSCC tissues and cells. RT-qPCR was conducted to determine the expression levels of miR-4712-5p. PTEN expression was detected by western blot assay and immunohistochemical analysis. (A) mRNA expression levels of PTEN in 56 paired human VSCC samples and adjacent normal tissues; (B) PTEN mRNA expression levels in VSCC cells and VECs; (C) PTEN expression in VSCC tissues and adjacent normal tissues of 8 clinicopathological tissue samples via western blot assay; (D) PTEN expression in VSCC tissues and adjacent normal tissues via immunohistochemistry (magnification ×200, scale bar=100 µm); *P<0.05. PTEN, phosphatase and tensin homologue; VSCC, vulvar squamous cell carcinoma; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; VECs, vaginal epithelial cells; N, normal.