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. 2019 Oct 4;7:254. doi: 10.3389/fbioe.2019.00254

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Copyright © 2019 Eichmann, Oberpaul, Weidner, Gerlach and Czermak.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Lucimycin production in V. natriegens. (A) Western blot (anti-GFP antibody) of soluble ssYahJ-His-Trx-GFP-lucimycin in the periplasmic (PP), cytoplasmic (CP), and insoluble and membrane (I) fraction 4 and 6 h post-induction. The upper black arrow indicates the expected band at 53.2 kDa, the lower arrow indicates the putative mature protein after the cleavage of the secretion signal. (B) Fusion protein quantification in the periplasmic and cytoplasmic fractions 2, 4, and 6 h post-induction. Error bars indicate the standard deviation.