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. 2019 Aug 7;14(12):2118–2125. doi: 10.4103/1673-5374.262592

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Copyright: © Neural Regeneration Research

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Effect of growth arrest-specific 5 (GAS5) on cell proliferation and differentiation.

(A, C) 5-Ethynyl-2′-deoxyuridine (EdU) immunofluorescence labeling was used to detect cell proliferation. The lncRNA GAS5-overexpressing lentivirus (LV-GAS5) group had a lower ratio of EdU-positive cells compared with the other two groups. (B) Tuj1 immunopositivity (red, stained by Alexa Fluor568) in different groups. Bars: 200 μm. (D) The ratio of Tuj1-positive cells in the LV-GAS5 group was higher than in control and negative control lentivirus (LV-NC) groups. (E) Average lengths of Tuj1-positive cell processes in the LV-GAS5 group were markedly longer than observed in either LV-NC or control groups. Data are expressed as the mean ± SEM. **P < 0.01, ***P < 0.001 (one-way analysis of variance followed by independent sample t-test). The experiment was repeated three times.