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. 2019 Oct 4;10:2272. doi: 10.3389/fmicb.2019.02272

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Copyright © 2019 Qian, Zhang, Jia, Sun, Zhang, Xiao and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The isolated PEDV strain JS-A was identified. (A) Simulated infected Vero cells of 24 hpi (a) and negative control Vero cells at 24 hpi (b). PEDV-infected (c) or negative control Vero cells (d) were examined by IFA at 24 hpi using polyclonal antibodies against PEDV N protein. (B) PEDV-infected or negative control Vero cells were subjected to analysis at 24 hpi with polyclonal antibodies against PEDV N protein by western blot; (C) electron microscopic images of purified PEDV particles were analyzed.