Table 1.
Primers for amplifications of the PEDV genomic fragments by RT-PCR.
| Name | Sequence (5′ to 3′) | Position in PEDV genome | Size (bp) |
|---|---|---|---|
| PP1F | GTGGAATTTCATTAGGTTTG | 123-142 | 1,380 |
| PP1R | AAGCTTACGTATGAACCAAG | 1502-1483 | |
| PP2F | TGCTGGTCATGTTGTTGTTG | 1421-1440 | 1,488 |
| PP2R | TAGAAAGCGAAGCCATCAAC | 2908-2889 | |
| PP3F | ATTGAAACTTCTTTTGTGGA | 2817-2836 | 1,451 |
| PP3R | TTCTCATTTGCAGCATTAAC | 4267-4248 | |
| PP4F | CTGCTCTCTCCTTGGATTCT | 3940-3959 | 1,478 |
| PP4R | AGTATGGTCTAGCATGTGGA | 5417-5398 | |
| PP5F | TGTCACAGACAACAAACCTG | 5300-5319 | 1,487 |
| PP5R | CATCACCAAAGACATCAAAA | 6786-6767 | |
| PP6F | ATTGGTAATGTGATGCCTTT | 6609-6628 | 1,323 |
| PP6R | AAAGCTTAGTGCAAAGAAAG | 7931-7912 | |
| PP7F | TTTCAAAGGTTAAGAAATTCT | 7861-7881 | 1,408 |
| PP7R | CTCACAGTGGGTGGTGTGTAT | 9268-9248 | |
| PP8F | CGTTATACACACCACCCACT | 9244-9263 | 1,304 |
| PP8R | GGTAACAACAAAGCACACAA | 10547-10528 | |
| PP9F | CAGAGCATTTTGATTACCAT | 10465-10484 | 1,470 |
| PP9R | CAACTATGCCATCTCCTTCT | 11934-11915 | |
| PP10F | GGTGTGAGCGTATTGTTAAG | 11842-11861 | 1,266 |
| PP10R | AATGCATAGACACGATGAAT | 13107-13088 | |
| PP11F | TTTGATTAAGGTAGGTGCTT | 13012-13031 | 1,397 |
| PP11R | CAACACCATCAATAAGGTTC | 14408-14389 | |
| PP12F | TATGGTGGTTGGGACAATAT | 14363-14382 | 946 |
| PP12R | CTTCCAAAAGTGTGACAGAA | 15308-15289 | |
| PP13F | ATTGCTTGAACGTTATGTGT | 15142-15161 | 1,676 |
| PP13R | CATTACCCTTGCAAAAGATC | 16817-16798 | |
| PP14F | TTAAGCCTGATGTCTTCTTG | 16674-16693 | 1,691 |
| PP14R | CCTACAGCGAGTATCAAAAC | 18364-18345 | |
| PP15F | TGGACATGTATCCAGAATTT | 18312-18331 | 1,455 |
| PP15R | GCATGGAATAACCACACTTC | 19766-19747 | |
| PP16F | AAAATGTGGAGGTGGATGTT | 19670-19689 | 1,362 |
| PP16R | GGCCCAATGTTTTATTATCG | 21031-21012 | |
| S-1F | TAAGTTGCTAGTGCGTAAT | 20572-20590 | 1,692 |
| S-1R | GTCAACACAGAAAGAACTA | 22263-22245 | |
| S-2F | TGAGTCATGAACAGCCAA | 22003-22020 | 1,511 |
| S-2R | CTGGTTGCGCTGTAGAA | 23513-23497 | |
| S-3F | GCGTCTCTCATAGGTGGT | 23491-23508 | 1,310 |
| S-3R | GTCCAAGAAACATCACTG | 24801-24784 | |
| ORF3·EF | CGTGCAGTGATGTTTCTTGGAC | 24780-24801 | 895 |
| ORF3·ER | TTATACGTCAATAACAGTACT | 25674-25654 | |
| EMF | CTTCACTTGTCACCGGTTGT | 25557-25576 | 1,097 |
| EMR | CCTCAGTACGAGTCCTATAAC | 26549-26529 | |
| MF | GGCGAATTCAATATGTCTAACGGTTC | 25670-25695 | 675 |
| MR | CGCGTCGACCCATAAAGTTTCTGTT | 26368-26362 | |
| NF | GGATCCATGGCTTCTGTCAGCTTT | 26107-26218 | 1,332 |
| NR | GACGTCACATTGTTTAATTTCCTGTACC | ||
| 3F | GGAAAAGAAGAACAAGCGT | 27355-27373 | 580 |
| 3R | ATCGGTCACCAGTATTTTTTTTTTTTTTT |