Table 3.
Primers used for RT-qPCR analysis of genes expression of pig intestinal mucosa.
| Gene name | Primer sequence (5′−3′) | Amplicon length (bp) | Accession number |
|---|---|---|---|
| GAPDH-F | GGAAAGGCCATCACCATCTT | 85 | XM_021091114.1 |
| GAPDH-R | CATGGTCGTGAAGACACCAG | ||
| FcRn-F FcRn-R TLR2-F |
GGCGACGAGCACCACTACTG AGCCGACCATGATTCCAACC GAGTCTGCCACAACTCAAAGA |
88 68 |
HQ026019.1 XM_005653576.3 |
| TLR2-R TLR3-F |
CAGAACTGACAACATGGGTAGAA GCGGTCCTGTTCAGTTTCT | 72 | KT735340.1 |
| TLR3-R TLR4-F |
AAGGCATCTGCTGGGATTT AACTGCAGGTGCTGGATTTAT | 74 | AB078418.1 |
| TLR4-R TLR7-F |
CCGTCAGTATCAAGGTGGAAAG CCCAGGTCCTCGAATCATTAC | 77 | DQ647699.2 |
| TLR7-R TLR8-F |
CATTAAGAGGCAAGGAGGAAGA CTTTGATGATGACGCTGCTTTC | 77 | KF019635.1 |
| TLR8-R TLR9-F |
GGTGTGTCACTCCTGCTATTC CCTCACACATCTCTCACTCAAG | 82 | KC860785.1 |
| TLR9-R RIG-I-F |
GGTGACAATGTGGTTGTAGGA GAGCCCTTGTGGATGCTTTA | 91 | KC011279.1 |
| RIG-I-R | GGGTCATCCCTATGTTCTGATTC | ||
| TRIF-F | CTCCGGTGCAGTCAAACA | 91 | KC969185.1 |
| TRIF-R | GGTAGTGTGTGCTGGTTTCT | ||
| MyD88A-F | GGCAGCTGGAACAGACCAA | 41 | EU056736.1 |
| MyD88A-R | GGCAGGACATCTCGGTCAGA | ||
| MyD88B-F | TGCAGGTGCCCATCAGAAG | 64 | EU056737.1 |
| MyD88B-R | TGATGAACCGCAGGATGCT | ||
| NF-κB1(p105)-F | GAGGTGCATCTGACGTATTC | 118 | NM_001048232.1 |
| NF-κB1(p105)-R | CACATCTCCTGTCACTGCAT | ||
| NF-κB1(p50)-F | AAGCACGGAACTGTAGACAC | 107 | KC316024.1 |
| NF-κB1(p50)-R | TCTGTGGTTTCTGTGACTTTCC | ||
| RELA-F | ACATGGACTTCTCAGCCCTTCTGA | 285 | CN155798.1 |
| RELA-R | CCGAAGACATCACCCAAAGATGCT | ||
| TRAF6-F | GGGAACGATACGCCTTACAA | 156 | NM_001105286.1 |
| TRAF6-R | CTCTGTCTTAGGGCGTCC |