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. 2019 Oct 4;10:2312. doi: 10.3389/fimmu.2019.02312

Figure 2.

Figure 2

Protein expression of MC4R in MS lesions. (A) Double immunofluorescence labeling shows co-localization of MC4R (green) with GFAP-positive astrocytes (red). scale-bar = 5 μm. Nuclei were stained in blue (DAPI). (B) Immunostainings of representative control white matter, NAWM, an active lesion, and inactive lesion center. Active MS lesions are characterized by loss of proteolipid protein (PLP) and presence of MHCII positive leukocytes, while inactive lesions are characterized by a demyelinated core, with little presence of MHCII+ cells. MC4R is expressed in control and NAWM and increased immunoreactivity is observed in active lesions (open arrowheads, scale-bar = 25 μm). (C) Quantitative analysis (one-way ANOVA) of the immunoreactive area (% of total area) for MC4R in active lesions and chronic inactive lesion centers compared to NAWM and control (n = 3–5 per group). Data represent mean ± SEM. *p < 0.05.