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Setmelanotide time-dependently induces CREB phosphorylation. Representative western blots of MC4R+ astrocytes treated with setmelanotide (10 μM), lysed at 15, 30, and 60 min after stimulation. α-Tubulin was used as a protein loading control for both pCREB and CREB. Quantification of CREB phosphorylation relative to total CREB revealed significantly increased phosphorylation of CREB after setmelanotide stimulation. n = 3 independent experiments. One-way ANOVA. *p < 0.05, **p < 0.01.
