Table 1. Chemical composition, cultivable microbial population and alpha diversity of bacterial community of raw materials before ensiling.
| Item | S1 | S2 | P value |
|---|---|---|---|
| Chemical composition | |||
| DM% | 23.81 ± 1.12 | 29.63 ± 0.53 | 0.0012 |
| CP (%DM) | 22.62 ± 0.35 | 25.34 ± 0.21 | 0.0003 |
| NDF (%DM) | 38.39 ± 0.02 | 33.35 ± 0.28 | 0.00001 |
| ADF (%DM) | 29.73 ± 0.05 | 25.54 ± 0.4 | 0.0001 |
| WSC (%DM) | 1.54 ± 0.12 | 1.8 ± 0.05 | 0.0219 |
| Cultivable microbial population | |||
| Yeast (log10 cfu/g FM) | 4.38 ± 0.03 | 4.08 ± 0.01 | 0.00005 |
| Mold (log10 cfu/g FM) | 4.2 ± 0.01 | 3.9 ± 0.12 | 0.0099 |
| Alpha diversity of bacterial community | |||
| Observed species | 249.67 ± 17.21 | 175 ± 19.29 | 0.0075 |
| Shannon | 1.94 ± 0.46 | 1 ± 0.02 | 0.0244 |
| Simpson | 0.33 ± 0.05 | 0.61 ± 0.02 | 0.0006 |
| ACE | 327.64 ± 61.36 | 262.24 ± 15.8 | 0.1483 |
| Chao 1 | 309.88 ± 28.96 | 241.81 ± 7.24 | 0.0168 |
| Coverage | >0.99 | >0.99 | NA |
Notes.
- FM
- fresh material
- DM
- dry matter
- CP
- crude protein
- NDF
- neutral detergent fiber
- ADF
- acid detergent fiber
- WSC
- water soluble carbohydrates
- LAB
- lactic acid bacteria
- CFU
- colony forming units
- NA
- not applicable