Table 4. Fermentation characteristic and chemical composition in alfalfa silage.
| Items | LA | AA | LA/AA | BA | DM | CP | NH3-N | NDF | ADF | WSC |
|---|---|---|---|---|---|---|---|---|---|---|
| S1 group | ||||||||||
| CK | 2.8 ± 0.13d | 5.3 ± 0.047a | 0.52 ± 0.028d | 2.6 ± 0.11a | 20 ± 0.094e | 18 ± 0.05f | 1.8 ± 0.014a | 38 ± 0.089a | 30 ± 0.095a | 0.58 ± 0.012e |
| CE | 9.8 ± 0.24a | 5.3 ± 0.057a | 1.8 ± 0.026b | 1.2 ± 0.011b | 20 ± 0.078d | 21 ± 0.15e | 0.95 ± 0.026b | 32 ± 0.023d | 25 ± 0.035d | 0.64 ± 0.0088d |
| LF | 6.3 ± 0.36b | 4.7 ± 0.048b | 1.3 ± 0.065c | 0.84 ± 0.06d | 21 ± 0.084d | 21 ± 0.066d | 0.88 ± 0.018c | 34 ± 0.068b | 27 ± 0.071b | 0.52 ± 0.015f |
| LF+CE | 11 ± 0.31a | 4.2 ± 0.039c | 2.5 ± 0.067a | 1 ± 0.068c | 23 ± 0.27c | 25 ± 0.05a | 0.57 ± 0.026de | 28 ± 0.038g | 22 ± 0.076f | 0.98 ± 0.015b |
| S2 group | ||||||||||
| CK | 1.8 ± 0.2e | 2.9 ± 0.023f | 0.62 ± 0.074d | 0.57 ± 0.018e | 27 ± 0.14b | 22 ± 0.043c | 0.53 ± 0.012e | 33 ± 0.084c | 26 ± 0.032c | 1.2 ± 0.018a |
| CE | 5.9 ± 0.38b | 3.6 ± 0.031d | 1.6 ± 0.099b | 0.54 ± 0.012e | 27 ± 0.24b | 24 ± 0.044b | 0.59 ± 0.019d | 29 ± 0.19e | 23 ± 0.09e | 1.2 ± 0.015a |
| LF | 4 ± 0.2c | 3.2 ± 0.058e | 1.2 ± 0.074c | 0.58 ± 0.028e | 27 ± 0.34b | 22 ± 0.077c | 0.47 ± 0.0063f | 34 ± 0.27bc | 26 ± 0.26c | 0.79 ± 0.0088c |
| LF+CE | 6.7 ± 0.21b | 2.9 ± 0.011f | 2.4 ± 0.083a | 1 ± 0.0028c | 30 ± 0.09a | 25 ± 0.061a | 0.38 ± 0.01g | 29 ± 0.16f | 23 ± 0.055e | 1.2 ± 0.0088a |
| SEM | 0.38 | 0.06 | 0.097 | 0.073 | 0.27 | 0.11 | 0.025 | 0.2 | 0.16 | 0.018 |
| Main effects1 | ||||||||||
| WC | <0.001 | <0.001 | 0.103 | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 |
| A | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 |
| WC ×A | <0.001 | <0.001 | 0.193 | <0.001 | 0.002 | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 |
Notes.
- LA
- lactic acid
- AA
- acetic acid
- LA/AA
- the ratio of lactic acid and acetic acid
- BA
- butyric acid
- NH 3 -N
- ammonia-N
- DM
- dry matter
- CP
- crude protein
- NDF
- neutral detergent fiber
- ADF
- acid detergent fiber
- WSC
- water soluble carbohydrates
- LAB
- lactic acid bacteria
- FM
- fresh matter
- S1
- Sample1 (fresh)
- S2
- Sample 2 (wilted in the field for 5 h)
- CK
- untreated control
- CE
- application of commercial cellulose
- LF
- application of L. fermentum 17SD-2
- LF+CE
- combination of L. fermentum 17SD-2 and commercial CE
- SEM
- Standard error of mean
- ND
- not detected
- NA
- Not applicable
- 1. WC
- water content
- A
- Additives
- WC × A
- the interaction between water content and additives