Figure 7.

Lactobacillus gasseri prevents the production of TNF and IL-6 by inhibiting ADAM17 (a disintegrin and metalloproteinase 17). The gene expression and protein levels of ADAM17 were examined after incubation of THP-1-derived macrophages or MDMs with Hp or in combination with L. gas or L. bre. (A) mRNA levels of ADAM17 and (B) protein levels of ADAM17 in THP-1-derived macrophages were determined using qPCR or ELISA after 8 h of infection, respectively. (C) mRNA levels of ADAM17 and (D) protein levels of ADAM17 in MDMs were quantified using qPCR or ELISA at 8 h postinfection, respectively. (E) Protein levels of TNF in THP-1-derived macrophages were determined using ELISA after incubation of cells with or without TAPI-1 (50 μM). ^*P < 0.05. Data are the means and standard deviation of triplicate samples and are representative of three independent experiments. NS, non-significant.