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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Curr Protoc Cytom. 2019 Jul 17;90(1):e63. doi: 10.1002/cpcy.63

Figure 6: Establishing the detection sensitivity of MFC for MM MRD monitoring.

Figure 6:

MFC is a relevant tool that can be used for MM MRD monitoring. Prior to the implementation of the assay for clinical utility, the sensitivity of the assay needs to be established. For the determination of LLOQ, a bone marrow sample from a MM patient with a known level of PCs was serially dilution and processed as per procedural steps detailed in SUPPORT PROTOCOL 2. The resulting theoretical sensitivity (represented by dashed line) is established and the actual percentage of detected PCs (represented by solid line) is calculated for each dilution tested in the experiment. The experiment was repeated twice, error bars represent the standard deviation of these measurements.