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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Curr Protoc Cytom. 2019 Jul 17;90(1):e63. doi: 10.1002/cpcy.63

Table 3.

Immunophenotypic definition of the negative and positive populations used for calculating Stain Index.

Tested
Markers		 Negative Population
 Positive Population
Phenotype Generic Name Phenotype Generic Name
CD45 CD45-, SSClo Erythroid Precursors CD19-, CD56-, CD45br, SSClo T Cells
CD19 CD45br, CD56+, SSClo NK Cells CD19+, CD45br, SSClo B Cells
CD27 CD45+, CD117br Mast Cells CD19-, CD56-, CD45br, SSClo T Cells
CD81 CD45dim,SSChi Granulocytes CD19-, CD56-, CD45br, SSClo T Cells
CD56 CD19+, CD56-, CD45br, SSClo B Cells CD19-, CD56+, CD45br, SSClo NK Cells
CD117 CD19-, CD56-, CD45br, SSClo T Cells CD27, CD117br Mast Cells
CD138 CD19+, CD56-, CD45br, SSClo B Cells CD45dim, CD81+ Spiked Controla
CD38 CD19+, CD81dim Mature B CD19+, CD81+ B-progenitors
cKappa CD19+, CD45br, cLambda+, SSClo cLambda+ B Cells CD19+, CD45br, cLambda-, SSClo cLambda- B Cells
cLambda CD19+, CD45br, cKappa+, SSClo cKappa+ B cells CD19+, CD45br, cKappa-, SSClo cKappa- B cells
a

A procedural control for immunophenotyping, CD-Chex CD103™ Plus (Streck, Omaha, NE), which also expresses CD138 is spiked into the bone marrow sample and used as the positive population. Modified from (Soh et al., 2017)