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. 2019 Oct 11;15(10):e1008279. doi: 10.1371/journal.pgen.1008279

Fig 5. Analysis of promoter activity and transcription factor binding site in porcine fibroblast cells.

Fig 5

(A) Schematic overview of the XM_013981330.2:g.−1805_−1810del luciferase reporter constructs and the results of the promoter activity assay. The arrow represents the sequence segment location of the XM_013981330.2:g.−1805_−1810del. Data histograms and error bars represent the mean±standard error of triplicate independent samples. *P<0.05, **P<0.01. (B) ChIP assay of the binding of MRFs to the XM_013981330.2:g.−1805_−1810del promoter region in porcine fibroblast cells derived from KNPs (Q) and Landrace (q). (C) Results from cotransfection experiments in porcine fibroblast cells. The MYH3 q promoter acts as a stronger repressor than the MYH3 Q promoter. The 5DG4 represents a cotransfection experiment with all four MRFs. Data histograms and error bars represent the mean±standard error of triplicate independent samples. *P<0.05, **P<0.01.