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. Author manuscript; available in PMC: 2019 Oct 11.
Published in final edited form as: Breast Cancer Res Treat. 2017 Nov 28;168(2):543–550. doi: 10.1007/s10549-017-4595-8

Fig. 3.

Fig. 3

RT-PCR analysis demonstrates BRCA1 c.5332+4delA leads to exon 21 skipping. a RT-PCR products run on QIAxcel Advanced System from QIAGEN. An extra band was observed in the patient, but not in controls. b Electropherogram from ABI 3730xl DNA analyzer showing that the variant causes exon 21 skipping. The boundary of exons is marked by red arrow. c Semi-quantitative fragment analysis of RNA transcripts from the patient and controls