Figure 1.

Identification of NgR1 interacting proteins by Nogo-A stimulation. A, Twenty-one DIV cortical neurons were stimulated without or with Nogo22 (100 nm) for 5 min and then the lysate was immunoprecipitated with anti-NgR1 antibody. The immunoprecipitates were resolved by SDS-PAGE and visualized by silver staining. Black arrow bands were excised and analyzed by LC/MS to determine their identity. Red arrow shows immunoprecipitated NgR1 protein band. B, List of proteins determined their identity by LC/MS. C, D, NgR1 immunoprecipitates from 21 DIV cortical neurons stimulated without or with Nogo22 (100 nm) for 5 min were resolved by SDS-PAGE and immunoblotted for CRMP2, CRMP4 (C), and Myh10, Jup, and NgR1 (D). The graphs show the quantification of each protein levels in the immunoprecipitates normalized to total cell lysate from CRMP2 (n = 3), CRMP4 (n = 3), Myh10 (n = 4), and Jup (n = 4). Mean ± SE. *p < 0.05, ***p < 0.005, Student's two-tailed t test.