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. 2019 Apr 24;39(17):3332–3344. doi: 10.1523/JNEUROSCI.3498-17.2019

Figure 1.

Figure 1.

Development of a sensitive mouse progranulin ELISA. A, Design of a sandwich ELISA to detect mouse progranulin (GRN). B, Representative standard curve for the GRN ELISA. C, Measurement of serum GRN level from a WT or a Grn knock-out (KO) mouse. D, Measurement of extracellular GRN levels in supernatants from undifferentiated N2a cells, differentiated N2a cells, or 15 d in vitro mouse primary cortical neuron cultures. E, Identification of controls for the siRNA screen. Shown are measurements of extracellular GRN levels in supernatants from cultured differentiated N2a cells transfected with either 50 nm Scr nontargeting siRNA, 50 nm Pdia4 siRNA, or 50 nm Grn siRNA. ***p < 0.001; ****p < 0.0001.