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. 2019 Apr 24;39(17):3249–3263. doi: 10.1523/JNEUROSCI.2944-18.2019

Figure 1.

Figure 1.

Assessing the direction of Cre-recombinase to Oxtr in the PFC. A, Schematic illustrating (left) bilateral injections of the AAV-ChR2-eYFP into the PFC of Oxtr-Cre mice, (right, top) the viral construct used to specifically target OXTR-expressing neurons, and (right, bottom) the Cre-mediated inversion of the eYFP and ChR2 sequence that results in the expression of these genes. B, Unilateral coronal brain section through the PFC of an Oxtr-Cre mouse delivered AAV-ChR2-eYFP, demonstrating the specific transfection of PFC neurons (eYFP, green) relative to all neurons (HuC/D, red). C, A projection image of an eYFP-labeled (green) neuron residing in the PFC, (D) Oxtr mRNA (magenta), (E) merged image, and (F) pie graph showing the frequency by which eYFP-labeled PFC neurons also express Oxtr mRNA. G, Representative unilateral coronal section through the PFC demonstrating GCaMP6f fluorescent intensity before, and then (H) after bath application of 200 nm oxytocin. I, Average change in GCaMP6f fluorescent intensity following application of 200 nm oxytocin. Scale bars: B, 500 μm; C–E, 10 μm; G, H, 100 μm.