Figure 8.

sAPPα priming of LTP is dependent on the synthesis and trafficking of new proteins. a, Bath application of BFA (35 μm, n = 9) beginning 10 min before sAPPα application and continuing until sAPPα (1 nm, n = 8) washout prevented the facilitation of LTP observed in response to a TBS delivered 30 min later and measured 2 h after TBS (p = 0.009). The initial induction LTP for the BFA group was not significantly different from the sAPPα or the control group. b, Bath application of CHX (60 μm) also prevented the facilitation of LTP persistence (p = 0.009) and reduced the initial induction of LTP (n = 6) compared with the sAPPα group (n = 8, p = 0.022). c, Summary histogram showing the facilitation of LTP by sAPPα and the inhibition of the effect by both BFA and CHX. All data are shown as mean percentage change ± SEM. Dunnett's post hoc tests, *p < 0.05, **p < 0.01 compared with sAPPα alone treatment. Waveforms in a and b are as in previous figures. These data, in combination with the receptor subunit trafficking data, indicate that the priming mechanism of sAPPα involved a coordinated enhancement of protein synthesis and trafficking of glutamate receptors to the cell membrane.