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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: J Neurochem. 2019 Jul 28;151(1):50–63. doi: 10.1111/jnc.14813

Figure 7. Aspirin induces the recruitment of PPARα to the Socs3 gene promotor.

Figure 7.

A) MAP of the proximal PPRE in the Socs3 gene promoter and the region to be amplified for ChIP assay. Primary astrocytes isolated from WT mice were treated with different concentrations of aspirin for 1 h under serum-free condition followed by monitoring the recruitment of PPARα, PPARβ PPARγ, and RNA Polymerase II to the proximal PPRE by ChIP assay (B, PCR; C, real-time PCR). Results are mean ± SD of three independent cell preparations. ***p < 0.001 vs control. Significance of mean between control and aspirin-treated cells was analyzed by a two-tailed paired t-test. D) MAP of the distal PPRE in the Socs3 gene promoter and the region to be amplified for ChIP assay after aspirin treatment. ChIP assay was performed to monitor the recruitment of PPARα, PPARβ PPARγ, and RNA Polymerase II to the distal PPRE (E, PCR). We did not find any amplification.