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. Author manuscript; available in PMC: 2020 Oct 11.
Published before final editing as: Matrix Biol. 2019 Apr 11:S0945-053X(18)30483-9. doi: 10.1016/j.matbio.2019.04.001

Figure 6: Biowire II platform can generate high fidelity tissues using different hydrogels.

Figure 6:

BJ1D iPSC derived ventricular tissues were electrically stimulated as shown in panel A). Quantitative comparison of B) Excitation Threshold (ET), (n⩾8), C) Maximum Capture Rate (MCR), (n⩾8), D) Percent of tissues successfully reaching a positive Force-Frequency Relationship (FFR) by the end of cultivation (two batches of tissues), E) FFR, F) Post-Rest Potentiation (PRP) for tissues generated using Collagen vs. Collagen/Fibrin hydrogel (n⩾7). Data presented as mean ± stdev, Student’s t-test or two-way ANOVA with Tukey’s multiple comparisons test. G) Confocal images of tissues generated from Collagen vs. Collagen/Fibrin hydrogel stained with for α-actinin, myosin light chain-2v (MLC2v) and F-actin stain, and counterstained with the nuclear stain DAPI (left panels); and stained for connexin-43 (Cx43) and cardiac troponin T (cTNT), counterstained with DAPI (right panels). Scale bar=30 μm. H) Quantification of myofiber alignment using cTNT staining. (n=3). Data presented as mean ± stdev, Student’s t-test or Mann-Whitney test or ANOVA on ranks with Dunn’s multiple comparisons test.