Fig. 2.

JHU37152 and JHU37160 exhibit high in vivo DREADD occupancy. a Structures of JHU37152 (J52) and JHU37160 (J60). b Brain and serum concentrations and ratios of J52 and J60 in mice (n = 4 mice per condition) at different time points after a 0.1 mg kg⁻¹ (IP) injection. C21 (1 mg kg⁻¹, IP) data are same as shown in Supplementary Figures for comparison purposes. c, d J52 and J60 (0.1 mg kg⁻¹, IP) displace in vivo [¹¹C]clozapine binding to DREADDs in AAV-DREADD-expressing mice (n = 5 mice). e, f J60 (0.1 mg kg⁻¹, IP) selectively blocks in vivo [¹¹C]clozapine binding to DREADDs in rats (n = 3 rats). g, h J60 (0.1 mg kg⁻¹) blocks in vivo [¹¹C]clozapine binding to hM4Di in the monkey. All data represented are mean ± SEM except in (h) where individual values are displayed. Source data are provided as a Source Data file