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. 2019 Oct 11;10:4627. doi: 10.1038/s41467-019-12236-z

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© This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — [¹⁸F]JHU37107 enables noninvasive detection of DREADD in locally-targeted cells and at their long-range projections. a Structure of [¹⁸F]JHU37107. b–d [¹⁸F]JHU37107 selectively binds to DREADDs in the brain of transgenic D1-DREADD mice (n = 3 mice per condition) and is blocked by 0.1 mg kg⁻¹ of JHU37160. e–j [¹⁸F]JHU37107 selectively binds to AAV-DREADDs expressed in the rat cortex and enables noninvasive and longitudinal mapping of both local (injection site) and long-range projections of motor cortex circuitry (ventrolateral thalamus shown as a main hub). Representative immunohistochemical images showing GFP (green) or HA-tagged DREADDs (red) from representative rats are shown side by side with their corresponding [¹⁸F]JHU37107 PET images. The white arrows point at corresponding anatomical regions. k–m [¹⁸F]JHU37107 binds to hM4Di expressed in the monkey amygdala and at putative projection sites. All data are represented as mean ± SEM except in (l) and (m) where individual values are displayed. Scale bars are 1 mm. Source data are provided as a Source Data file and the raw PET data are available upon request