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. 2019 Oct 11;9:14692. doi: 10.1038/s41598-019-51198-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Relative position of amplification primers and probes along the fungal ribosomal genes. The relative location of two amplicons and five fungal biomarker target sequences are displayed. Pan-fungal primer set ITS3 and LR1 and pan-Candida primer set LR0 and GSCand are depicted as arrows. FAM-labelled pan-fungal, C. krusei-specific, C. glabrata-specific and pan-Candida probes are marked with a green circle; the HEX-labelled C. auris-specific probe is marked with an orange circle. Quencher molecules are shown as black circles. Not drawn to scale.